cryo-EM - 2025 NAFTA

Packet 8: Bonus 4

Improper loading of protein samples during this technique may lead to the resolution of “leopard skin” patterns. For 10 points each:

[10m] Name this technique whose “single-particle” variant aligns homogenous samples using cross-correlation. Dubochet, Frank, and Henderson were awarded a Nobel Prize in 2017 for developing this technique.

ANSWER: cryo-EM [or cryo-electron microscopy or electron cryomicroscopy or cryo-TEM or ECT or electron cryo-tomography or cryo-electron tomography or cryo-ET or CET; prompt on scanning electron microscopy or transmission electron microscopy; prompt on microscopy]

[10h] In 2020, this protein’s structure was reconstructed at a 1.22 angstrom resolution using single-particle cryo-EM. Proteins named for resembling this hollow 24-subunit protein can be encased by family 1 encapsulins.

ANSWER: ferritin [or apoferritin; accept bacterioferritin; accept ferritin-like proteins; prompt on FLPs]

[10e] Since 2022, single-particle cryo-EM has been used to assess the viability of ferritin-based D·C·F·H·P vaccines targeting this pathogen with delta and omicron variants.

ANSWER: COVID-19 virus [or SARS-CoV-2 or severe acute respiratory syndrome coronavirus 2; accept coronavirus disease 2019; prompt on SARS-CoV or coronavirus or covid]

<MS, Biology> | NAFTA-Packet-8

Heard	PPB	E %	M %	H %
43	13.02	100%	30%	0%

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Conversion

Team	Opponent	Part 1	Part 3	Total	Parts
1000000 Delinquents	(give me an) ANSWER: die for baseball	0	10	10	E
A Neg of Beauty is a Joy Forever	H Charlson's Washed Up Scrubs	0	10	10	E
Abbas I founded the Abbasids	UBC B	0	10	10	E
Ana Pauker? I hardly know her!	The Umayyad Caliphate be like REPEAL NAPHTHA	10	10	20	ME
Berkeley A	Stanford B	0	10	10	E
Berkeley B	Team 7	10	10	20	ME
C R A B S	Dour Straits	0	10	10	E
Constans Constantius and Constantine Jr.	Stanford A	10	10	20	ME
Contessa Dora	Catholic University A	0	10	10	E
Copán Seethe	It's something unpredictable/that in the end is right/please refrain from e-mailing my wife	10	10	20	ME
Dielectrical Materialism	Vacuous Tortoise	0	10	10	E
Execute Order 6 7	BHSU	0	10	10	E
Georgia Tech 𓂈𓆉𓃡	Say It Ain't Zhou	10	10	20	ME
Habitat 67	Noah Sheidlower	10	10	20	ME
I'm just here (being trivia club president) so I won't get fined	I fear not the man who has reviewed 10000 cards once, but I fear the man who has reviewed one card 10000 times	0	10	10	E
In the Mood for Transportation Equipment	Chicago !A	0	10	10	E
Indiana	Muscle Mass Extinction Event	0	10	10	E
JHU	Mike and Psmith	10	10	20	ME
Lex	WashU	0	10	10	E
Licence to C. Wright Mill	Licence to Bill	0	10	10	E
Maryland F♯ A♯ ∞	https://i.imgur.com/C6nN2FE.png	10	10	20	ME
Not Old! (Old)	Team 8	0	10	10	E
Penn State	Rosslyn Academic Team	0	10	10	E
Polish Orthographers Posting Their Łs	David Foster Gromit	0	10	10	E
Quebecois Olga Tokarczuk be like 'freins de jacob interdits'	Northwestern	10	10	20	ME
Shipping Down from Ballston	Six Quizbowlers in Search of Owen Minmo	10	10	20	ME
Strange Case of Dr. Joker and Mr. Pennywise	You Don't Have the Cards	0	10	10	E
Surrealist Subcommandante Marcos be like Esto no es una pipa	Capital One in the Twenty-First Century	10	10	20	ME
Tennessee A	Girls who Just Want to Have Fun + Liam	0	10	10	E
The "C" Team	NYU B or not NYU B (that is the question)	0	10	10	E
The Fire Neg Time	consumption smoothing by borrowing future big law income	0	10	10	E
The Saint-Straw that Broke the Camille's Back	Middlesex County Fetty Wap Fan Club	0	10	10	E
The Third Time when she was Given to Answer the Hard and the Easy, and Answered Only the Easy	No Country for Old Quizzers	0	10	10	E
The Worst Trade Deal in the History of Trade Deals	Maybe Ever	10	10	20	ME
The lion does not concern itself with negging teammates out of their own categories	Where's the nearest library, I need to have a public crashout.	0	10	10	E
The tram is not in service; please use the moving walkway.	Invective Against Swans	0	10	10	E
UBC A	The Unbearable Heaviness of History Players	0	10	10	E
UNC Charity	Munir Siddiqui Fan Club	0	10	10	E
Untitled Keyal Team	NYU A	10	10	20	ME
Untitled by Alexander McQueen by Premeditated Chaos by Alexander McQueen by Percival Everett	Negged Five Times in a Yellow Cab Against the Gottis and Gambinos	0	10	10	E
W&L+Friend	four high school generalists shock natives with fluent stock in Tocharian B	0	10	10	E
William of Baskerville attempts to investigate John P. Robarts, gets turned away for not having a student card	Guelph Cloze	0	10	10	E
[Redacted]	St. Marx	0	10	10	E

Summary

Tournament	Edition	Match	Heard	PPB	E %	M %	H %
2026 NAFTA at Stanford	01/17/2026	✓	4	15.00	100%	50%	0%
2026 NAFTA at UBC	01/17/2026	✓	2	10.00	100%	0%	0%
2025 NAFTA Online	02/14/2026	✓	4	12.50	100%	25%	0%
2026 NAFTA at Vanderbilt	02/14/2026	✓	3	16.67	100%	67%	0%
2025 NAFTA at Toronto	09/13/2025	✓	5	10.00	100%	0%	0%
2025 NAFTA at Maryland	09/27/2025	✓	5	14.00	100%	40%	0%
2025 NAFTA at Harvard	10/04/2025	✓	3	13.33	100%	33%	0%
2025 NAFTA at Oxford	10/11/2025	✓	4	10.00	100%	0%	0%
2025 NAFTA at Chicago	11/08/2025	✓	6	11.67	100%	17%	0%
2025 NAFTA at Columbia	11/08/2025	✓	5	16.00	100%	60%	0%
2025 NAFTA at Richmond	12/20/2025	✓	2	15.00	100%	50%	0%